Blood Culture Collection

General · General · 10–20 min

Aseptic collection of blood cultures for suspected bacteremia.

Indications

  • Suspected sepsis / endocarditis / FUO

Equipment

  • Aerobic + anaerobic bottles
  • Chlorhexidine 2%
  • Needle & syringe / butterfly with adapter

Positioning

  • As appropriate for the procedure and patient comfort.

Consent

  • Explain indication, benefits, alternatives
  • Explain risks & complications
  • Obtain written/verbal consent as per SOP
  • Document consent

Universal precautions

  • Hand hygiene
  • PPE — gloves, mask, eye protection
  • Aseptic technique
  • Sharps safety
  • Verify patient identity & site

Step-by-step (0/5)

Tap to complete

1. Prep site

Chlorhexidine 30s, dry 30s.

2. Clean bottle top

3. Draw 20 mL

4. Two sets from different sites

5. Label & transport

Immediate complications

  • Contamination — false positive

Recognition

  • Skin flora growth

Initial management

  • Repeat cultures

Prevention

  • Aseptic technique
  • Correct volume

Post-procedure observation

  • Vitals q15min × 1h then hourly

Monitoring

  • Site inspection
  • Symptom review

Documentation

  • Procedure note
  • Consent
  • Findings

Patient instructions

  • Report pain, bleeding, fever, worsening symptoms

Educational bedside guide. Perform only under supervision until competent. Follow local SOPs and consent policy.

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